Antioxidants Cause Inflammation

The human body has multiple ways of eliminating the hydrogen peroxide that is generated during metabolism. Glutathione and Thioredoxin rely on a steady stream of NADPH to eliminate the peroxide. This causes the NAD+/NADH pool to become more oxidized due to NADPH being regenerated from NADH by the mitochondrial enzyme NNT.

In short, peroxide removal by glutathione decreases the amount of NADH and the NADH/NAD+ ratio. Another way to say that is that ROS removal by glutathione eliminates reductive stress.

On the other hand, catalase is a magical enzyme that can eliminate ROS without needing to rely on any co-factors such as NADPH. The end result is that using catalase, you can eliminate ROS without affecting the NADH/NAD+ redox balance.

Interestingly, evolution decided not to use catalase in the mitochondira, relying instead on glutathione and thioredoxin, both of which oxidize NADPH.

I wondered what would happen if catalase was overexpressed in the mitochondria. Would it have real world consequences? Would it push up mitochondrial NADH?

The answer to both questions is yes.

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Catalase Causes Inflammation

In the paper “Enhanced Expression of Catalase in Mitochondria Modulates NF-κB–Dependent Lung Inflammation through Alteration of Metabolic Activity in Macrophages”, the authors did exactly what I’ve suggested. They overexpressed catalase in mouse mitochondria, meaning that hydrogen peroxide was being eliminated by a combination of catalase and glutathione, rather than purely by glutathione. They stimulated the mice lungs with LPS (lipopolysaccharide) from e. coli. LPS stimulates an inflammatory response. The body uses ROS to fight bacterial infection.

The catalase eliminated a lot of peroxide:

Overexpressing catalase (black bars) helped reduce the oxidative stress caused by LPS.

Using catalase rather than glutathione/thioredoxin resulted in a more reduced NAD pool:

Catalase increased NADH/NAD+ levels in cells whether or not they were stimulated with LPS.

The animals who overexpressed catalase experienced increased inflammation. Mice with catalase “had exaggerated NF-κB activation in the lungs, increased neutrophilic alveolitis, and greater lung inflammation/injury” despite the fact that they had reduced oxidative stress. This is noteworthy because NF-KB has long been known to be activated by oxidative stress.

On the other hand, NF-KB is known to be activated by acetylation. Acetylation is caused by reductive stress: a buildup of acetyl-CoA (which does the acetylating) and NADH (which inhibits Sirt1 DEacetylase).

This paper nicely demonstrates which process is truly driving inflammation. Overexpressed mitochondrial catalase lowers oxidative stress and increases reductive stress in response to LPS challenge. The catalase causes increased inflammation.

Reductive stress causes inflammation.

Just Double Checking!

To ensure that reductive stress was truly the thing driving inflammation, the authors took cells from the mice and cultured them with lactate – which increases the NADH/NAD+ ratio. The lactate increased markers of inflammation.

Lactate increases NADH levels and NFkB activity. It also increased inflammatory markers (not shown).

Conversely, they incubated cells with a pharmaceutical that reduced the NADH/NAD+ ratio and NFkB activity and inflammation were decreased:

Reducing NADH levels reduces inflammation

Conclusions

Inflammation is increased by high NADH/NAD+ levels: reductive stress. This is presumably due to acetylation of NFkB. A good way to give yourself reductive stress is to use antioxidants that don’t oxidize the NAD pool.

In case you’re wondering: Vitamin C and Vitamin E DO reduce the NADH pool.


Han, W., Fessel, J. P., Sherrill, T., Kocurek, E. G., Yull, F. E., & Blackwell, T. S. (2020). Enhanced Expression of Catalase in Mitochondria Modulates NF-κB–Dependent Lung Inflammation through Alteration of Metabolic Activity in Macrophages. In The Journal of Immunology (Vol. 205, Issue 4, pp. 1125–1134). The American Association of Immunologists. https://doi.org/10.4049/jimmunol.1900820

Greene, W. C., & Chen, L.-F. (2008). Regulation of NF-κB Action by Reversible Acetylation. In Reversible Protein Acetylation (pp. 208–222). John Wiley & Sons, Ltd. https://doi.org/10.1002/0470862637.ch15

Schreck, R., Albermann, K., & Baeuerle, P. A. (1992). Nuclear Factor Kb: An Oxidative Stress-Responsive Transcription Factor of Eukaryotic Cells (A Review). In Free Radical Research Communications (Vol. 17, Issue 4, pp. 221–237). Informa UK Limited. https://doi.org/10.3109/10715769209079515

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13 thoughts on “Antioxidants Cause Inflammation”

  1. Hi Brad, I came to wonder why you went away from the feasting mimicking, rolling 48h fasts?

    I am preparing to attempt it myself, but I came to wonder if you stopped it because it was detrimental somehow?

    1. I stooped first of all because it was sort of hard to maintain in the context of socializing, etc. I also have a philisophical argument that we SHOULD be able to lose weight while eating in a normalish pattern. I’m trying to prove that. As to fasting, clearly a lot of people benefit from it, but I DO have my concerns. Fasting is how animals enter torpor, for instance.

        1. That’s a brilliant question..

          I think hibernating animals are the only to really fast, and they exit torpor by burning up the PUFA.
          I assume temperature also has something to do with it.

    1. It should. SUpposedly it creates a small but continuous stream of mitochondrial ROS. I haven’t used it in a long time but it’s interesting.

  2. so does taking a massive amount of glutathione precursors (N-Acetyl Cysteine) increase reductive stress and make it harder to lose weight?

  3. I’m very curious if you think increasing metabolism and increasing lactate dehydrogenase could produce more oxalates in the body. I have been losing weight with the pyruvate and carnitine but I’m now having a few joint issues I have not had for years. It looks as though metabolism could possibly produce oxalates? It looks like If adequate amounts of vitamin B6 are available, the enzyme AGT converts glyoxylate to the amino acid glycine. If glyoxylate isn’t converted to glycine then lactate dehydrogenase will convert it to oxalate. Does this make sense? I’m going to increase B6, really eliminate any oxalate food (i dont eat hardly any just chocolate) and take DMSA for a few days and see if my joint pain decreases. Curious to know if you think this could be happening with increased metabolic rate?

      1. Yeah you’re right, I should have took more time looking at that. I appreciate the work you’re doing. It has helped me piece together why pufas are so bad and completely shifted my thinking on blood glucose.

      2. Must be the lactate because if I stop for a day or two all of the muscle soreness goes away. When I start it comes back. Anything to clear lactate faster?

  4. I think overexpressing catalase or glutathion peroxidase is even more dangerous, because it switches off cell protective mechanism provided by expressing of HIF-1 through H2O2 and disable cell from switch on glucose to lactate metabolism and excretetion of unusable fuel as lactate. This view can also be simplified as overfeeding cell by fuel. H2O2 is the regulator of right fuel intake. I wonder, instead of fasting peaple we schuld more concentrate on how to fast cells.

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